Wavenumber, cm⁻¹

Functional group

Assignment

8192

CH_2-

C-H stretch 2nd overtone

5901

CH_3-

C-H stretch 1st overtone

5839

CH_3-

C-H stretch 1st overtone

5785

CH_2-

C-H stretch 1st overtone

5715

-SH

S-H 1st overtone

5577

O-H

Water

5191

-CONH

C=O 1st overtone

4659

HC-CH

=C-H +C=C

4350

Protein

C-H 2nd overtone

4288

Protein

C-H +C-H def

4227

Protein

C-H def 2nd overtone

4150

Fat

C-H +C-C

4042

CH₂

C-H